Did you know that Jesse James was murdered by one of his own gang?

Confirming the death of America’s most infamous outlaw

December 17th, 1869, Jesse James robbed a bank in Gallatin, Missouri and killed the cashier. While it was far from his first act of violence, this event changed the course of his life forever. In the days following the robbery, authorities officially declared him an outlaw, and offered rewards for the capture of Jesse and his brother Frank. Jesse eluded capture for many more years, before a fellow gang member fatally shot him in a bid to claim the reward money. But there was always speculation that another man was killed in his place, until DNA testing finally put those rumours to rest.

Jesse Woodson James was many things – an American outlaw, a guerrilla, a bank robber, a train robber and the leader of the James-Younger gang. Jesse’s life of crime may have been precipitated by his brother Frank’s involvement in the American civil war, since it’s said that a teenage Jesse was ambushed at their farm by Union militiamen looking for Frank. At the age of 16, Jesse followed his brother’s footsteps and joined the “bushwacker” group of Pro-Confederate guerrillas, and was involved in many atrocities and murders. After the war ended, Jesse and Frank took part in numerous bank and train robberies as members of several gangs. Some claim Jesse was a modern day Robin Hood, but there was no evidence at all to show that he “stole from the rich and gave to the poor”.

Even with a $10,000 price tag of his head and at least two life-threatening chest injuries, Jesse managed to evade capture and death until 1882, when he was killed by Robert Ford, a member of his own gang. His death was far from that at the end of a Wild West movie. In fact, he was shot in the back of his head, while dusting a picture in his own living room. As the story goes, Robert had previously arranged with the governor of Missouri to take down Jesse in exchange for the reward payout. Hence, Robert and his brother Charley were pardoned from hanging even after they were convicted.

Drawing depicting the murder of Jesse James
The murder of Jesse James

The public was transfixed by Jesse’s death, and the Ford brothers were said to have re-enacted the events of his death in a travelling show. But even that didn’t put a stop to the rumours that another man had died in his place, and his supposed death was all a scam to allow Jesse to escape justice. One man, J. Frank Dalton, who claimed to be Jesse, even had several injuries (bullet wounds, a collapsed lung, damaged fingertip and burned feet) that resembled the characteristics of the real Jesse. However, his recollection of earlier life events did not stack up when questioned by Jesse’s relatives.

More than a 100 years later, scientists sought to put to rest the speculation surrounding the death of Jesse using DNA tests. They exhumed the presumptive remains of Jesse from the grave at Mt Olivet Cemetery where his body had been reburied in 1902. Samples were also taken from his original grave at the James’ family farm. Mitochondrial DNA (mtDNA) was the DNA type of choice for the genetic analysis, as it is strictly maternally inherited (from mother to child), so remains unchanged from generation to generation through the maternal line. Blood samples were taken from two maternal descendants of Jesse’s sister Susan to use as references, as these two individuals would carry the same mtDNA profile as Jesse.

DNA Maternal Ancestry Test box

DNA Maternal Ancestry Test

Three regions of the mtDNA genome can be analyzed – the coding region and two non-coding regions called HVR1 and HVR2. Often the sequence of just HVR1 is all that is required to confirm or refute potential maternal relationships. An identical HVR1 sequence was obtained from the two molar teeth from the reburial site and from the hair samples from the original burial site, indicating that they came from the same individual. This sequence was also an exact match to the profile obtained from the two reference samples, confirming that the remains buried at the James’ farm, and subsequently moved to the Mt Olivet Cemetery, belonged to an individual from the same maternal line as Jesse. Of course, it’s possible that these remains belonged to another maternal relative, rather than Jesse. However, given the scientific evidence and historical records, it’s very likely that Jesse was indeed murdered in his own home in 1882.

Maybe you choose to believe that Jesse lived an interesting life, in an ordinary house, in an ordinary town, as depicted by Brad Pitt in the film “The Assassination of Jesse James by the Coward Robert Ford”. Or maybe you think that Robert Ford did America a favour by stopping this infamous outlaw. Regardless of your opinion, we now know the mtDNA of Jesse James, and if you have taken the DNA Maternal Ancestry Test, you can see if you may have descended from the same maternal lineage as this infamous American outlaw.


Stone AC et al. (2001) Mitochondrial DNA Analysis of the Presumptive Remains of Jesse James. J Forensic Sciences. 46(1):173-176.

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